C. Perfringens NA Protein (N-His, C.perfringens)

Product Specifications

Abbreviation

C. Perfringens NA

Host

E. coli

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a fluorogenic substrate, 2'- (4-Methylumbelliferyl) -α-D-N-acetylneuraminic acid. The specific activity is 150,000 pmoles/min/μg (Activity of this protein is much better than the world's famous brand) as measured under the described conditions .

Function

Cys2-Gln382

Molecular Weight

The recombinant C. Perfringens neuraminidase consisting of 388 amino acids and has a calculated molecular mass of 43.6 kDa. It migrates as an approximately 40 kDa in SDS-PAGE under reducing conditions.