CD3E Protein (C-His, Human)

1.Immobilized SP34, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3e / CD3 epsilon Protein (His Tag) (Cat: 10977-H08S), the EC50 is 4.5-13.5 ng/mL. 2.Loaded anti-CD3E antibody on ProA Biosensor, can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08S) with an affinity constant of 4.33 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Mouse Anti-Human CD3 (SP34) captured on Protein A chip can bind Recombinant Human CD3 epsilon/CD3e Protein, His Tag (Cat. No. 10977-H08S) with an affinity constant of 1.419 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human CD3E consists of 115 amino acids and predicts a molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 23.3 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 33.3 kDa verified by SEC-MALS (Routinely tested) .