Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant SARS-CoV-2 BA.1 Spike RBD Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H23-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H), the EC50 is 1.2-4 ng/mL (QC tested) .

Function

Arg319-Lys529

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 237 amino acids and predicts a molecular mass of 27.24 kDa. As a result of glycosylation, it migrates as an approximately 36.9 kDa band in SDS-PAGE under reducing conditions.