TNFR1/TNFRSF1A Protein (C-His, Mouse)
Product Specifications
Abbreviation
TNFR1/TNFRSF1A
Synonyms
CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI, TNFRp55, TNFalpha-R1, Tnfr-2, Tnfr1, p55, p55-R
Host
HEK 293
Tag
C-His
Applications
Cell culture, ELISA
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 96 % as determined by reducing SDS-PAGE.
Bioactivity
1. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL. 2. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL. 3. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1. 5 μg/mL in the presence of 0.1 ng/mL recombinant mouse TNFα.
Function
Met 1-Ala 212
Molecular Weight
21.8 kDa
Species
Mouse
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
