HtrA2/Omi Protein (C-His, Human)

HTRA2, High temperature requirement protein A2, HtrA2, OMI, Omi stress-regulated endoprotease, PRSS25, Serine protease 25, Serine protease HTRA2, Serine proteinase OMI, mitochondrial

Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905) . Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in > 95% cleavage of β-casein, as revealed by SDS-PAGE.