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E.coli mdh Recombinant Protein

E. coli mdh Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-312aa. Application (s) : Enzyme Activity, SDS-PAGE.

Product Specifications

Synonyms

Malate dehydrogenase, ECK3225, JW3205

Gene Name

Mdh

UniProt

P61889

Tag

His-Tag

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE

Field of Research

Metabolism

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

34.9 kDa (336aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl

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