Mouse DKK-1 PicoKine® Quick ELISA Kit

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 97 101 103 99 2.59 2.6% Sample 2 425 361 363 388 384 25.82 6.7% Sample 3 1418 1320 1228 1302 1317 67.74 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 97 101 103 99 2.59 2.6% Sample 1 97 97 101 103 99 2.59 2.6% Sample 2 425 361 363 388 384 25.82 6.7% Sample 2 425 361 363 388 384 25.82 6.7% Sample 3 1418 1320 1228 1302 1317 67.74 5.1% Sample 3 1418 1320 1228 1302 1317 67.74 5.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 425 1418 94 417 1519 Standard deviation 6.4 30.17 68.06 7.4%9 30.85 88.1 CV (%) 6.6% 7.1% 4.8% 6.8% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 425 1418 94 417 1519 Standard deviation 6.4 30.17 68.06 7.4%9 30.85 88.1 CV (%) 6.6% 7.1% 4.8% 6.8% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 425 1418 94 417 1519 Mean (pg/ml) 97 425 1418 94 417 1519 Standard deviation 6.4 30.17 68.06 7.4%9 30.85 88.1 Standard deviation 6.4 30.17 68.06 7.4%9 30.85 88.1 CV (%) 6.6% 7.1% 4.8% 6.8% 7.4% 5.8% CV (%) 6.6% 7.1% 4.8% 6.8% 7.4% 5.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.043 0.095 0.160 0.289 0.551 1.028 1.756 2.063 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.043 0.095 0.160 0.289 0.551 1.028 1.756 2.063 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.043 0.095 0.160 0.289 0.551 1.028 1.756 2.063 O.D. 0.043 0.095 0.160 0.289 0.551 1.028 1.756 2.063

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.