Human Properdin / Complement Factor P ELISA Kit PicoKine®

Human Properdin / Complement Factor P ELISA Kit PicoKine® (96 Tests) . Quantitate Human CFP in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealternative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 616 576 554 549 573 26.42 4.6% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 3 9062 9597 10032 10437 9782 510.91 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 616 576 554 549 573 26.42 4.6% Sample 1 616 576 554 549 573 26.42 4.6% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 2 2125 2256 2211 2286 2219 60.73 2.7% Sample 3 9062 9597 10032 10437 9782 510.91 5.2% Sample 3 9062 9597 10032 10437 9782 510.91 5.2%

Synonyms

Properdin; Complement factor P; CFP; PFC

Gene Name

CFP

Gene ID

5199

UniProt

P27918

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D28-L469

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Microbiology, Protein

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 616 2125 9062 602 2285 9683 Mean (pg/ml) 616 2125 9062 602 2285 9683 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 Standard deviation 28.33 91.37 561.84 30.1 109.68 629.39 CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5% CV (%) 4.6% 4.3% 6.2% 5% 4.8% 6.5%

Sensitivity

<10 pg/mL

Function

A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Properdin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Complement factor properdin

Subcellular Location

Secreted.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052 O.D. 0.026 0.08 0.119 0.215 0.374 0.716 1.249 2.052

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.