Human Complement Factor H/CFH ELISA Kit PicoKine®

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation') . This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 3 53844 53833 53094 52680 53362 497.77 0.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 1 3203 2994 3170 3050 3104 85.4 2.7% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 2 13518 12944 13467 11714 12910 726.54 5.6% Sample 3 53844 53833 53094 52680 53362 497.77 0.9% Sample 3 53844 53833 53094 52680 53362 497.77 0.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Mean (pg/ml) 3203 13518 53844 3406 12935 57149 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 Standard deviation 233.81 784.04 3822.92 303.13 905.45 4971.96 CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7% CV (%) 7.3% 5.8% 7.1% 8.9% 7% 8.7%

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519 O.D. 0.031 0.094 0.157 0.295 0.487 1.032 1.901 2.519

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.