Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit

Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Complement Factor H/CFH in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation') . This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1344 1258 1323 1429 1339 84.33 6.3% Sample 2 6741 6541 5974 6641 6474 407.88 6.3% Sample 3 29641 26474 24165 26852 26783 1553.41 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1344 1258 1323 1429 1339 84.33 6.3% Sample 1 1344 1258 1323 1429 1339 84.33 6.3% Sample 2 6741 6541 5974 6641 6474 407.88 6.3% Sample 2 6741 6541 5974 6641 6474 407.88 6.3% Sample 3 29641 26474 24165 26852 26783 1553.41 5.8% Sample 3 29641 26474 24165 26852 26783 1553.41 5.8%

Gene Name

Cfh

Gene ID

12628

UniProt

P06909

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S875-V1252

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1344 6741 29641 1352 6941 26413 Standard deviation 72.58 242.68 1304.2 51.38 423.4 1558.37 CV (%) 5.4% 3.6% 4.4% 3.8% 6.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1344 6741 29641 1352 6941 26413 Standard deviation 72.58 242.68 1304.2 51.38 423.4 1558.37 CV (%) 5.4% 3.6% 4.4% 3.8% 6.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1344 6741 29641 1352 6941 26413 Mean (pg/ml) 1344 6741 29641 1352 6941 26413 Standard deviation 72.58 242.68 1304.2 51.38 423.4 1558.37 Standard deviation 72.58 242.68 1304.2 51.38 423.4 1558.37 CV (%) 5.4% 3.6% 4.4% 3.8% 6.1% 5.9% CV (%) 5.4% 3.6% 4.4% 3.8% 6.1% 5.9%

Sensitivity

<20 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Cfh

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.002 0.313 0.609 1.052 1.626 2.217 2.290 2.478 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.002 0.313 0.609 1.052 1.626 2.217 2.290 2.478 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.002 0.313 0.609 1.052 1.626 2.217 2.290 2.478 O.D. 0.002 0.313 0.609 1.052 1.626 2.217 2.290 2.478

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.