Blue Tetrazolium

Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF) . Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH) [1].

Product Specifications

CAS Number

[1871-22-3]

Product Name Alternative

Tetrazolium blue

UNSPSC

12352200

Hazard Statement

H350

Target

Fluorescent Dye

Related Pathways

Others

Field of Research

Others

Solubility

10 mM in DMSO

Smiles

COC(C=C(C1=CC(OC)=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)C=C1)C=C5)=C5N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]

Molecular Formula

C40H32Cl2N8O2

Molecular Weight

727.64

Precautions

H350

References & Citations

[1]Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46 (4) : 376-380.