BCECF Acetoxymethyl ester

BCECF Acetoxymethyl ester is a fluorescent indicator for estimating intracellular pH. BCECF Acetoxymethyl ester is modified by intracellular esterases to produce BCECF. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm[1][2][3].

Product Specifications

CAS Number

[1119449-23-8]

UNSPSC

12352005

Target

Fluorescent Dye

Related Pathways

Others

Field of Research

Others

Smiles

O=C(C1=CC=C2C(C(OC23C4=C(OC5=C3C=C(CCC(OCOC(C)=O)=O)C(OCOC=O)=C5)C=C(OCOC(C)=O)C(CCC(OCOC(C)=O)=O)=C4)=O)=C1)OCOC(C)=O

Molecular Formula

C41H38O21

Molecular Weight

866.73

References & Citations

[1]Bachmeier CJ, et al. Drug efflux transport properties of 2',7'-bis (2-carboxyethyl) -5 (6) -carboxyfluorescein acetoxymethyl ester (BCECF-AM) and its fluorescent free acid, BCECF. J Pharm Sci. 2004 Apr;93 (4) :932-42.|[2]Dive C, et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol Cell Probes. 1988 Jun;2 (2) :131-45.|[3]O'Connor N, et al. Ratio imaging: practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol. 2007;81:415-33.