N3-CMC

N3-CMC is a click chemistry reagent. N3-CMC containing an azide group can undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups. N3-CMC can also undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. N3-CMC reacts specifically with the pseudouridine (Ψ) -containing RNA, and biotin is subsequently conjugated to the N3-CMC-Ψ RNA via click chemistry, quantitatively analyzing the dynamic changes of Ψ[1].