AMP-PNP (tetralithium)

AMP-PNP (Adenylyl-imidodiphosphate) tetralithium is a non-hydrolyzable ATP analog. AMP-PNP tetralithium binds to ATP binding sites competely but is not hydrolyzed by enzymes, providing stable experimental conditions for studying ATP-dependent processes. AMP-PNP tetralithium can also be used to study enzyme activity, kinase regulation, DNA/RNA metabolism, ion channel function, and protein complex assembly[1][2][3][4].

Product Specifications

CAS Number

[72957-42-7]

Product Name Alternative

Adenylyl-imidodiphosphate (tetralithium)

UNSPSC

12352005

Target

Potassium Channel

Related Pathways

Membrane Transporter/Ion Channel

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Metabolic Disease

Purity

97.0

Solubility

H2O : 26.5 mg/mL (ultrasonic; warming)

Smiles

O[C@H]1[C@H](N2C3=C(N=C2)C(N)=NC=N3)O[C@@H]([C@H]1O)COP(OP(NP(O[Li])(O[Li])=O)(O[Li])=O)(O[Li])=O

Molecular Formula

C10H13Li4N6O12P3

Molecular Weight

529.93

References & Citations

[1]Korkhov VM, et al. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature. 2012 Oct 18;490 (7420) :367-72.|[2]Sakharov PA, et al. Nonhydrolyzable ATP analog 5'-adenylyl-imidodiphosphate (AMP-PNP tetralithium) does not inhibit ATP-dependent scanning of leader sequence of mRNA. Biochemistry (Mosc) . 2015 Jan;80 (1) :45-9.|[3]Ellinghaus TL, et al. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021 Dec 24;7 (52) :eabk2392.|[4]Hehl S, Neumcke B. KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP tetralithium. Eur Biophys J. 1994;23 (4) :231-7.