BCECF-Acetoxyemnethy ester

BCECF-Acetoxyemnethy ester is a cell membrane permeable compound widely-used as a fluorescent indicator for intracellular pH. BCECF-Acetoxyemnethy ester could diffuse through the cell membrane and intracellular esterase cleave the ester bond releasing BCECF (HY-101882) . BCECF allows measurements in the physiological pH range 6.0-8.0. Excitation ratio: 490/440 nm; Emission intensity: 535 nm[1].

Product Specifications

UNSPSC

12171500

Target

Fluorescent Dye

Related Pathways

Others

Field of Research

Others

Concentration

5 mM * 50 μL in DMSO

Purity

95.0

Smiles

O=C(OCOC(C)=O)CCC1=CC(C23OC(C4=C2C=C(C(OCOC(C)=O)=O)C=C4)=O)=C(OC5=C3C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C5)C=C1OCOC(C)=O.O=C(OCOC(C)=O)CCC6=CC(C78OC(C9=C7C=CC(C(OCOC(C)=O)=O)=C9)=O)=C(OC%10=C8C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C%10)C=C6OCOC(C)=O

Molecular Formula

C42H40O21

Molecular Weight

880.76

References & Citations

[1]Ozkan P, et al. A rapid method for measuring intracellular pH using BCECF-AM. Biochim Biophys Acta. 2002 Aug 15;1572 (1) :143-8.|[2]Chen F, et al. CYP51A1 drives resistance to pH-dependent cell death in pancreatic cancer. Nat Commun. 2025 Mar 7;16 (1) :2278. |[3]Pang X, et al. Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration. Nat Commun. 2023 Jul 14;14 (1) :4207.