ProtoGel Quick-Cast 12%

Description Catalog number:EC-895Ideal for Western Blotting ApplicationsCast a 12% Gel in One Fourth the TimeEliminates Mixing and MeasuringPrecast Convenience without the Precast PriceFor even sharper bands and higher resolution, tryProtogel Quick-Cast Loading Buffer.  Specially formulated to give superior results on Quick Cast Gels.  Now your Quick-Cast gels give even better results!With the introduction of ProtoGel Quick-Cast, the time required for casting gels for Western Blots has been reduced from 2 hours to under 25 minutes, and the number of steps involved has been cut from 10 to only 3. With ProtoGel Quick-Cast, simply measure out the amount needed, add initiators and cast the gel. No mixing, no multiple measurements, no need for a second casting to produce a stacking gel.ProtoGel Quick-Cast gels are run in standard 1X Tris-Glycine SDS. Electrical parameters will vary from apparatus to apparatus, but typically gels are run at 170V for 60 minutes.Ideal for Western BlottingMouse liver protein (25mg) was resolved on either a 12% Protogel Quick Cast gel (1) or a 12% Laemlli gel (2) and blotted to PVDF. The respective blots were probed an Anti-GAPDH antibody @ 1:50,000 dilution and an HRP-conjugated secondary antibody @ 1:50,000 dilution. The blots were detected with ProtoGlow ECL substrate.ProtoGel Quick-Cast gels require no special handling after the run. Simply stain according to standard protocols.Storage: ProtoGel Quick-Cast is stable for 24 months when stored tightly capped in the refrigerator (40C).References:“PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention”Sheng Yaoet al.Genes & Cancer 2010 1: 444.  online at:http://gan.sagepub.com/content/1/5/444.full“Somatic coliphage families as potential indicators of enteric viruses in water and methods for their detection”Hee Suk Lee, PhD dissertation 2009, UNC Chapel Hill