CheKine™ Micro Trypsase Activity Assay Kit

Trypsase is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsase catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Trypsase is widely used in the treatment of local edema, hematoma and abscess due to the pyothorax, hemothorax, surgical inflammation, ulcer, traumatic injury, etc. CheKine TM Micro Trypsase Activity Assay Kit can be used to detect biological samples such as animal and plant tissues. In the kit, the ester bond of TAME was hydrolyzed by trypsase, and the free carboxyl released neutralized with sodium hydroxide in the reaction system, resulting in the decrease of pH value of the solution. The trypsase activity data can be quickly measured by using phenol red as an indicator to determine the change of the absorption value of the solution at 555 nm. There was a good linear relationship between trypsase and the decrease of absorption value at 555 nm in a certain range.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at ４°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.