Monoclonal Anti-T7 Tag
Product Specifications
Background
The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.
Host
Mouse
Reactivity
All
Immunogen
A synthetic peptideMASMTGGQQMG conjugated to KLH.
Applications
Western Blot (1:1000-10000) ; Immunofluorescence, IF (1:100-1:1000) ; Immunoprecipitation, IP (1:100-1:1000)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
References & Citations
1. Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189 (1) :113-30
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Isotype
IgG1
Curated Selection
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