Recombinant human alpha-Synuclein E46K protein

Alpha-Synuclein E46K is a mutant form of alpha-synuclein which is changed at position 46 from glutamate (E) to lysine (K) . Recent studies have shown that this mutant (E46K) of alpha-synuclein causes Parkinson and Lewy Body dementia (DLB) . alpha-Synuclein E46K was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques

Product Specifications

Product Name Alternative

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

14.4 (140aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

A-Synuclein E46K, SNCA, NACP, PARK1, alpha-Synuclein, alpha synuclein E46k, alpha-synuclein e46k, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Alpha synuclein, Alpha-synuclein isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Synuclein alpha, SNA4001-10 µg, SNA4001-20 µg, SNA4001-50 µg, SNA4001-100 µg, SNA4001-250 µg, SNA4001-500 µg, SNA4001-1 mg, SNA4001-10, SNA4001-20, SNA4001-50, SNA4001-100, SNA4001-250, SNA4001-500, SNA4001-1

References & Citations

Juan J.Zarranz, . (2004) Ann. Neurol. 55, 164-173.

Other References

Park JY, et al. Microglial phagocytosis is enhanced by monomeric alpha-synuclein, not aggregated alpha-synuclein: implications for Parkinsons disease. (Glia. 2008) {https://pubmed.ncbi.nlm.nih.gov/18449945/}; ; Kim KS, et al. Proteolytic cleavage of extracellular α-synuclein by plasmin: implications for Parkinson disease. (J Biol Chem. 2012) {https://pubmed.ncbi.nlm.nih.gov/22619171/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_000336.1

Uniprot Accession Number

P37840

Species

Human

AA Sequence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKKGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA