Recombinant human SETD7 protein

SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet) . The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET 7/9, unlike most other SET proteins, is exclusively a mono-methylase, Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Product Specifications

Product Name Alternative

SETD7, SET7, SET9, SET7/9, SET7/9 Histone methyltransferase, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.7 kDa (366aa) confirmed by MALDI-TOF

Additionnal Information

SETD7, SET9, SET7/9 Histone methyltransferase, SET7/9, SET7, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, SET domain containing protein 7, SET D7, SET 9, SET 7/9, SET 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43, SET3001-10 µg, SET3001-20 µg, SET3001-50 µg, SET3001-100 µg, SET3001-250 µg, SET3001-500 µg, SET3001-1 mg, SET3001-010, SET3001-020, SET3001-050, SET3001-100, SET3001-250, SET3001-500, SET3001-01M

References & Citations

Xiao B., et al. (2003) Nature. 421 (6923) :652-6.; ; Kwon T., et al. (2003) EMBO J. 22, 292-303.;

Other References

Choi KC, et al. Gallic acid suppresses lipopolysaccharide-induced nuclear factor-kappaB signaling by preventing RelA acetylation in A549 lung cancer cells. (Mol Cancer Res. 2009) {https://pubmed.ncbi.nlm.nih.gov/19996305/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 5mM DTT, 20%glycerol

Scientific Category

Histone Related

NCBI Accession Number

NP_085151

Uniprot Accession Number

Q8WTS6

Species

Human

AA Sequence

MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK