Recombinant E.coli dsbA protein

Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids) . Recombinant DsbA (residues 20-208) was expressed in E. coliand purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thiol disulfide interchange protein dsbA, rpbB, ppfA, dsf, DsDNA-binding protein A, dsbA, Double-stranded DNA-binding protein, Disulfide oxidoreductase A periplasmic protein disulfide isomerase I, Disulfide oxidoreductase A, Disulfide oxidoreductase (DsbA) E.coli

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.2 kDa (190aa) confirmed by MALDI-TOF

Additionnal Information

Disulfide oxidoreductase, DsbA, Disulfide oxidoreductase A, dsbA, rpbB, Disulfide oxidoreductase (DsbA) E Coli, DsDNA-binding protein A, Double-stranded DNA-binding protein, Disulfide oxidoreductase A periplasmic protein disulfide isomerase I, Thiol disulfide interchange protein dsbA., HSP0601-10 µg, HSP0601-20 µg, HSP0601-50 µg, HSP0601-100 µg, HSP0601-250 µg, HSP0601-500 µg, HSP0601-1 mg, HSP0601-10, HSP0601-20, HSP0601-50, HSP0601-100, HSP0601-250, HSP0601-500, HSP0601-1

References & Citations

Charbonnier, J. B. et al. (1999) . Protein Sci. 8:96-105; ; Inaba, K. et al. (2004) . J. Biol. Chem. 279:6761-6768.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_418297

Uniprot Accession Number

P0AEG4

Species

Human

AA Sequence

MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK