Recombinant human Vitronectin protein

Vitronectin, also known VTN, is a member of the pexin family. It is a cell adhesion and spreading factor found in serum and tissues. This protein interacts with glycosaminoglycans and proteoglycans and is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. It also contains endogenous cleavage sites such as elastase, thrombin and plasmin. This protein binds multiple ligands, including the soluble vitronectin receptor which affects multi-function. Therefore, it is involved in hemostasis, cell migration, as well as tumor malignancy. Recombinant human Vitronectin protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human VTN stimulates cell adhesion of the JB16-F1 mouse melanoma cells. This effect is more to 50%.

Product Name Alternative

VTN, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, Somatomedin B, Complement S-protein

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 0.1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When cells are added to human VTN coated plates 5 µg/mL. This effect is more to 50%.

Molecular Weight

53.3 kDa (468aa)

Additionnal Information

Vitronectin, VitronectinVitronectin, VTN, V75, VN, VNT, ATGP3849-10 µg, ATGP3849-20 µg, ATGP3849-50 µg, ATGP3849-100 µg, ATGP3849-250 µg, ATGP3849-500 µg, ATGP3849-1 mg, ATGP3849-10, ATGP3849-20, ATGP3849-50, ATGP3849-100, ATGP3849-250, ATGP3849-500, ATGP3849-1, ATGP4165-10 µg, ATGP4165-20 µg, ATGP4165-50 µg, ATGP4165-100 µg, ATGP4165-250 µg, ATGP4165-500 µg, ATGP4165-1 mg, ATGP4165-010, ATGP4165-020, ATGP4165-050, ATGP4165-100, ATGP4165-250, ATGP4165-500, ATGP4165-01M

References & Citations

Yang XP., et al, (2016) Cancer Biomark. 17:271-279.; ; Min SK., et al, (2017) Cell Death Differ. 25:268-281.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000629.3

Uniprot Accession Number

P04004

Species

Human

N Terminal Sequence

Ala

AA Sequence

DQESCKG RCTEGFNVDK KCQCDELCSY YQSCCTDYTA ECKPQVTRGD VFTMPEDEYT VYDDGEEKNN ATVHEQVGGP SLTSDLQAQS KGNPEQTPVL KPEEEAPAPE VGASKPEGID SRPETLHPGR PQPPAEEELC SGKPFDAFTD LKNGSLFAFR GQYCYELDEK AVRPGYPKLI RDVWGIEGPI DAAFTRINCQ GKTYLFKGSQ YWRFEDGVLD PDYPRNISDG FDGIPDNVDA ALALPAHSYS GRERVYFFKG KQYWEYQFQH QPSQEECEGS SLSAVFEHFA MMQRDSWEDI FELLFWGRTS AGTRQPQFIS RDWHGVPGQV DAAMAGRIYI SGMAPRPSLA KKQRFRHRNR KGYRSQRGHS RGRNQNSRRP SRATWLSLFS SEESNLGANN YDDYRMDWLV PATCEPIQSV FFFSGDKYYR VNLRTRRVDT VDPPYPRSIA QYWLGCPAPG HL