Recombinant human PlGF-1/PGF protein
Placenta growth factor (PlGF/PGF) is a member of the PDGF/VEGF-sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. There are three isoforms of this protein: PlGF-1, PlGF-2, PlGF-3. PlGF-2 is only found in early placenta up until the 8th week of development, while PlGF-1 is specifically found in the colon as well as mammary carcinomas. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. Also, PlGF is ultimately associated with angiogenesis. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Recombinant human PlGF-1/PGF, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human PGF/PLGF-1 is coated at 2 µg/mL (100 ul/well) can bind Human VEGFR1/Flt-1 (CAT# ATGP4101) in a Functional ELISA assay.
Product Name Alternative
Placenta growth factor, PlGF, PGF, PGFL, PLGF, placenta growth factor isoform 2
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human VEGFR1/Flt-1 (CAT# ATGP4101) .
Molecular Weight
15.5kDa (137aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular
NCBI Accession Number
NP_001193941.1
Uniprot Accession Number
P49763-2
Species
Human
N Terminal Sequence
Leu 19
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
