Recombinant human EphB1 protein
EphB1 also known as Ephrin type-B receptor 1, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors. EphB1 has been shown to bind ephrin-B2, ephrin-B1, ephrin‑A3, ephrin-A1, ephrin-A4, and ephrin-B3. It binds to tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. The ephrin/Eph families also appear to play a role in angiogenesis. Recombinant human EPHB1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human EPHB1 is coated at 2 µg/mL (100 ul/well) can bind Human EFNB1 (CAT# ATGP3800) in a Functional ELISA assay.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human EFNB1 (CAT# ATGP3800) .
Molecular Weight
59.2 kDa (529aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular
NCBI Accession Number
NP_004432.1
Uniprot Accession Number
P54762
Species
Human
N Terminal Sequence
Met18
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