Recombinant human NRG1/HRG1 protein

Neuregulin-1 (NRG1), also known as ARIA, is one of four proteins in the neuregulin family that act on the EGFRfamily of receptors. It has multiple isoforms that aregenerated by usage of different promoters and alternative splicing of a single gene. This protein regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination. In the heart, it regulates organ morphogenesis and contractility and also plays a cardioprotective role following tissue injury. NRG1 is essential for the development and function of multiple organ systems, and itsdysregulation has been linked to diseases such as cancer and schizophrenia. Recombinant human NRG1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatographytechniques.

Product Specifications

Specifications

Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 15 ng/mL.

Product Name Alternative

NRG1, ARIA, GGF2, GGF, GGFglial growth factor, HGL, HGLneu differentiation factor, HRG, HRG1, HRGA, NRG-IT2, NRG1-IT2, HRG1-alpha, HRG1-beta 1, HRGAneuregulin 1 type IV beta 1a, Heregulin-1, MST131, MSTP131, NDFheregulin, alpha (45kD, ERBB2 p185-activator), Neuregulin-1, Neuregulin1, SMDF, neuregulin 1, neuregulin 1 type IV beta 3, pro-NRG1, pro-neuregulin-1, membrane-bound isoform, sensory and motor neuron derived factor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 15 ng/mL.

Molecular Weight

25.1kDa (231aa)

Additionnal Information

, ATGP3990-10 µg, ATGP3990-20 µg, ATGP3990-50 µg, ATGP3990-100 µg, ATGP3990-250 µg, ATGP3990-500 µg, ATGP3990-1 mg, ATGP3990-10, ATGP3990-20, ATGP3990-50, ATGP3990-100, ATGP3990-250, ATGP3990-500, ATGP3990-1

References & Citations

Tan W, et al. (2007) . J Biol Chem. 282:24343-24351.; ; Nicodemus KK, et al. (2010) . Arch Gen Psychiatry. 67:991-1001.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_039252

Uniprot Accession Number

Q02297

Species

Human

N Terminal Sequence

Ala

AA Sequence

SGKKPES AAGSQSPALP PRLKEMKSQE SAAGSKLVLR CETSSEYSSL RFKWFKNGNE LNRKNKPQNI KIQKKPGKSE LRINKASLAD SGEYMCKVIS KLGNDSASAN ITIVESNEII TGMPASTEGA YVSSESPIRI SVSTEGANTS SSTSTSTTGT SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYSTSTPF LSLPE