Recombinant human ACE-2 protein
ACE-2, also known as Angiotensin-converting enzyme 2, is an enzyme attached to the cell membranes of cells in the lungs, arteries, heart, kidney, and intestines. This enzyme lowers blood pressure by catalysing the hydrolysis of angiotensin II into angiotensin. It counters the activity of the related angiotensin-converting enzyme (ACE) by reducing the amount of angiotensin-II and increasing Ang (1-7) making it a promising drug target for treating cardiovascular diseases. ACE-2 also serves as the entry point into cells for some coronaviruses, including HCoV-NL63, SARS-CoV, and SARS-CoV-2. Recombinant human ACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques
Product Specifications
Specifications
SARS-CoV-2 Spike RBD is coated at 5 µg/mL (100 ul/well) can bind Human ACE-2. The ED50 range ≤ 100 ng/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Enzyme Activity, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Molecular Weight
110.8kDa (965aa)
Additionnal Information
References & Citations
Hamming I., et al, (2004) J Pathol. 203:631-637.; ; Mohammed A.r. Chamsi-Pasha., et al, (2014) Curr Heart Fail Rep. 11:58-63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Virus
NCBI Accession Number
NP_068576.1
Uniprot Accession Number
Q9BYF1
Species
Human
N Terminal Sequence
Asp
AA Sequence
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