Recombinant Porcine GM-CSF protein
GM-CSF, also known as granulocyte macrophage colony-stimulating factor, is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant porcine GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Specifications
Porcine GM-CSF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/mL.
Product Name Alternative
Expression System
E.coli
Antigen Species
Pocrine
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/mL.
Molecular Weight
16.6 kDa (148aa), confirmed by MALDI-TOF
Additionnal Information
References & Citations
Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80. Wong GG, et al (1985) Science, 228, 810-815.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH7.4)
Scientific Category
Growth factors
NCBI Accession Number
NP_999283
Uniprot Accession Number
Q29118
Species
Pocrine
AA Sequence
APTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK
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