Recombinant human Complement Factor B protein

Complement factor B, also known as CFB, is a component of the alternative pathway of complement activation.Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, itis cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The activesubunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb isinvolved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. Recombinant human Complement factor B protein, fused to His-tag at C-terminus, was expressed in insect cell and purified byusing conventional chromatography techniques.

Product Specifications

Product Name Alternative

CFB, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, Properdin factor B, BF, BFD, AHUS4, ARMD14, CFAB, CFBD, FB, FBI12, GBG, H2-Bf

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

58.1 kDa (514aa)

Additionnal Information

Complement Factor B, ATGP3903-10 µg, ATGP3903-20 µg, ATGP3903-50 µg, ATGP3903-100 µg, ATGP3903-250 µg, ATGP3903-500 µg, ATGP3903-1 mg, ATGP3903-10, ATGP3903-20, ATGP3903-50, ATGP3903-100, ATGP3903-250, ATGP3903-500, ATGP3903-1

References & Citations

Francis PJ., et al. (2009) J Med Genet. 46:300-307.; ; Schwaeble W., et al (1993) . Immunobiology 188:221-232.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001701

Uniprot Accession Number

P00751

Species

Human

N Terminal Sequence

Ala

AA Sequence

KIVLDPS GSMNIYLVLD GSDSIGASNF TGAKKCLVNL IEKVASYGVK PRYGLVTYAT YPKIWVKVSE ADSSNADWVT KQLNEINYED HKLKSGTNTK KALQAVYSMM SWPDDVPPEG WNRTRHVIIL MTDGLHNMGG DPITVIDEIR DLLYIGKDRK NPREDYLDVY VFGVGPLVNQ VNINALASKK DNEQHVFKVK DMENLEDVFY QMIDESQSLS LCGMVWEHRK GTDYHKQPWQ AKISVIRPSK GHESCMGAVV SEYFVLTAAH CFTVDDKEHS IKVSVGGEKR DLEIEVVLFH PNYNINGKKE AGIPEFYDYD VALIKLKNKL KYGQTIRPIC LPCTEGTTRA LRLPPTTTCQ QQKEELLPAQ DIKALFVSEE EKKLTRKEVY IKNGDKKGSC ERDAQYAPGY DKVKDISEVV TPRFLCTGGV SPYADPNTCR GDSGGPLIVH KRSRFIQVGV ISWGVVDVCK NQKRQKQVPA HARDFHINLF QVLPWLKEKL QDEDLGFL