Recombinant mouse SPARC-like 1/SPARCL1 protein
SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Recombinant Mouse SPARCL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
71.7 kDa (642aa)
Additionnal Information
References & Citations
Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991-996.; ; Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735-748.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_034227
Uniprot Accession Number
P70663
Species
Mouse
N Terminal Sequence
Ile17
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