Recombinant human EphB4 protein

EPHB4, also known ephrin type-B receptor 4, is a member of the Eph receptor tyrosine kinase family. It binds transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. This protein plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. And the signaling of this protein controls cellular repulsion and segregation form EFNB2-expressing cells. And it also plays a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Recombinant human EPHB4 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ephrin type-B receptor 4, EPHB4, HFASD, HTK, MYK1, TYRO11, EPH receptor B4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

58.1 kDa (532aa)

Additionnal Information

EPHB4, Ephrin type-B receptor 4, EPHB4, HFASD, HTK, MYK1, TYRO11, ATGP3843-10 µg, ATGP3843-20 µg, ATGP3843-50 µg, ATGP3843-100 µg, ATGP3843-250 µg, ATGP3843-500 µg, ATGP3843-1 mg, ATGP3843-10, ATGP3843-20, ATGP3843-50, ATGP3843-100, ATGP3843-250, ATGP3843-500, ATGP3843-1

References & Citations

Liu T., et al, (2017) Cell Physiol Biochem. 41:819-834.; ; Kadife E., et al, (2018) Acta Oncol. 25:1-14.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_004435

Uniprot Accession Number

P54760

Species

Human

N Terminal Sequence

Leu16

AA Sequence

LEETLLNTKL ETADLKWVTF PQVDGQWEEL SGLDEEQHSV RTYEVCDVQR APGQAHWLRT GWVPRRGAVH VYATLRFTML ECLSLPRAGRSCKETFTVFY YESDADTATA LTPAWMENPY IKVDTVAAEH LTRKRPGAEA TGKVNVKTLR LGPLSKAGFY LAFQDQGACM ALLSLHLFYK KCAQLTVNLT RFPETVPREL VVPVAGSCVV DAVPAPGPSP SLYCREDGQW AEQPVTGCSC APGFEAAEGN TKCRACAQGT FKPLSGEGSC QPCPANSHSN TIGSAVCQCR VGYFRARTDP RGAPCTTPPS APRSVVSRLN GSSLHLEWSA PLESGGREDL TYALRCRECR PGGSCAPCGG DLTFDPGPRD LVEPWVVVRG LRPDFTYTFE VTALNGVSSL ATGPVPFEPV NVTTDREVPP AVSDIRVTRS SPSSLSLAWA VPRAPSGAVL DYEVKYHEKG AEGPSSVRFL KTSENRAELR GLKRGASYLV QVRARSEAGY GPFGQEHHSQ TQLDESEGWR EQLAVEHHHH HH