Recombinant mouse Prolactin protein
Prolactin, also known as prl, is a hormone synthesized and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland) . Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation) . Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immuno-regulation. Recombinant mouse Prolactin, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse Prolactin stimulates cell proliferation of the Nb2-11 rat lymphoma cells. The ED50 range ≤3 ng/mL.
Product Name Alternative
Prolactin isoform 1, AV290867, Gha1, Prl1a1
Expression System
E.coli
Antigen Species
Mouse
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED50 range ≤3 ng/mL.
Molecular Weight
22.7 kDa (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Suzuki MF., et al. (2012) Biotechnol Appl Biochem. 59 (3) :178-85; ; Goffin V., et al. (1999) Mol Cell Endocrinol. 151 (1-2) :79-87.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Apoptosis
NCBI Accession Number
NP_035294
Uniprot Accession Number
Q9CPQ2
Species
Mouse
AA Sequence
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