Recombinant mouse eIF-4E/EIF4E protein

Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, eIF-4F 25 kDa subunit

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.6 kDa (241aa) confirmed by MALDI-TOF

Additionnal Information

Eif4e, Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, ATGP3550-10 µg, ATGP3550-20 µg, ATGP3550-50 µg, ATGP3550-100 µg, ATGP3550-250 µg, ATGP3550-500 µg, ATGP3550-1 mg, ATGP3550-10, ATGP3550-20, ATGP3550-50, ATGP3550-100, ATGP3550-250, ATGP3550-500, ATGP3550-1

References & Citations

Piron F., et al. (2010) PLoS One. 5 (6) :e11313.; ; Cao R., et al. (2015) Nat neurosci. 18 (6) :855-62.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_031943.3

Uniprot Accession Number

P63073

Species

Mouse

AA Sequence

MATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V