Recombinant human CD46 protein

CD46, also known as membrane cofactor protein isoform 14, is a type 1 membrane protein that plays an important inhibitory role in the complement system. It is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome, a disease characterized by uncontrolled complement activation. Recombinant human CD46, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Membrane cofactor protein isoform 14, CD46, AHUS2, MCP, MIC10, TLX, TRA2.10

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.5 kDa (288aa)

Additionnal Information

CD46, Membrane cofactor protein isoform 14, CD46, AHUS2, MCP, MIC10, TLX, TRA2.10, ATGP3451-10 µg, ATGP3451-20 µg, ATGP3451-50 µg, ATGP3451-100 µg, ATGP3451-250 µg, ATGP3451-500 µg, ATGP3451-1 mg, ATGP3451-10, ATGP3451-20, ATGP3451-50, ATGP3451-100, ATGP3451-250, ATGP3451-500, ATGP3451-1

References & Citations

Lublin DM., et al. (1988) J Exp Med. 168:181-194.; ; Maga TK., et al. (2010) Hum. Mutat. 31:E1445-14460.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_758860

Uniprot Accession Number

P15529

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADLCEEPPTF EAMELIGKPK PYYEIGERVD YKCKKGYFYI PPLATHTICD RNHTWLPVSD DACYRETCPY IRDPLNGQAV PANGTYEFGY QMHFICNEGY YLIGEEILYC ELKGSVAIWS GKPPICEKVL CTPPPKIKNG KHTFSEVEVF EYLDAVTYSC DPAPGPDPFS LIGESTIYCG DNSVWSRAAP ECKVVKCRFP VVENGKQISG FGKKFYYKAT VMFECDKGFY LDGSDTIVCD SNSTWDPPVP KCLKGPRPTY KPPVSNYPGY PKPEEGILDS LDHHHHHH