Recombinant mouse EOGT protein

EOGT, also known as EGF domain-specific O-linked N-acetylglucosamine transferase, is involved in the regulation of Notch receptor. O-GlcNAc (O-linked b-N-acetylglucosamine) is introduced by a single intracellular O-GlcNAc transferase (OGT) and a single extracellular O-GlcNAc transferase (EOGT) . O-GlcNAc results from the addition of a single N-acetylglucosamine residue to serine/threonine residues. Recombinant mouse EOGT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, A130022J15RiK, Aer61, AI447490, AW214473, AW259391

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

60.4 kDa (516aa)

Additionnal Information

EOGT, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, A130022J15RiK, Aer61, AI447490, AW214473, AW259391, ATGP3328-10 µg, ATGP3328-20 µg, ATGP3328-50 µg, ATGP3328-100 µg, ATGP3328-250 µg, ATGP3328-500 µg, ATGP3328-1 mg, ATGP3328-10, ATGP3328-20, ATGP3328-50, ATGP3328-100, ATGP3328-250, ATGP3328-500, ATGP3328-1

References & Citations

Hart GW., et al. (2007) Nature 446:1017-1022.; ; Sakaidani Y., et al. (2012) Biochem Biophys Res Commun. 419:14-19.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_780522

Uniprot Accession Number

Q8BYW9

Species

Mouse

N Terminal Sequence

Asp20

AA Sequence

DKAHSEADDA PGKALYDYSS LRLPAEHIPF FLHNNRHVAS VCREDSHCPY KKHLENLNYC WGYEKSCAPE FRFGSPVCSY VDLGWTDTLE SAQDMFWRQA DFGYARERLG EIRTICQPER ASDSSLVCSR YLQYCRATGL YLDLRNIKRN HDRFKEDFLQ GGEIGGYCKL DSHALVSEGQ RKSPLQSWFA ELQGYTQLNF RPIEDAKCDI VVEKPTYFMK LDAGINMYHH FCDFLNLYLT QHVNNSFSTD VYIVMWDTST YGYGDLFSDT WKAFTDYDVI HLKTYDSKKV CFKEAVFSLL PRMRYGLFYN TPLISGCQNT GLFRAFSQHV LHRLNITQEG PKDGKVRVTI LARSTEYRKI LNQDELVNAL KTVSTFEVRV VDYKYRELGF LDQLRITHNT DIFIGMHGAG LTHLLFLPDW AAVFELYNCE DERCYLDLAR LRGIHYITWR KPSKVFPQDK GHHPTLGEHP KFTNYSFDVE EFMYLVLQAA EHVLQHPQWP FKKKHDELLE HHHHHH