Recombinant human CDK5RAP3 protein

CDK5RAP3 also known as CDK5 regulatory subunit-associated protein 3. This protein is potential regulator of CDK5 activity via its interaction with CDK5R. Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, nad an activating subunit, p25NCK5A. CDK5RAP3 binds to p25NCK5A and therefore may be involved in neuronal differentiation. Recombinant human CDK5RAP3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CDK5 regulatory subunit-associated protein 3 isoform b, C53, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114, PP1553

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

59kDa (526aa)

Additionnal Information

CDK5RAP3, CDK5 regulatory subunit-associated protein 3 isoform b, C53, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114, PP1553, ATGP3070-10 µg, ATGP3070-20 µg, ATGP3070-50 µg, ATGP3070-100 µg, ATGP3070-250 µg, ATGP3070-500 µg, ATGP3070-1 mg, ATGP3070-10, ATGP3070-20, ATGP3070-50, ATGP3070-100, ATGP3070-250, ATGP3070-500, ATGP3070-1

References & Citations

Kim S., et al. (2014) PLoS One. Jan 22; ;9 (1) :e87016.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_788276

Uniprot Accession Number

Q96JB5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR NQFLDELMEL EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT MVSVLEDLIG KLTSLQLQHL FMILASPRYV DRVTEFLQQK LKQSQLLALK KELMVQKQQE ALEEQAALEP KLDLLLEKTK ELQKLIEADI SKRYSGRPVN LMGTSL