Recombinant e.coli ackA protein

AckA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E. coli ackA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acetate kinase A and propionate kinase 2, ECK2290, JW2293

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

45.7 kDa (423aa) confirmed by MALDI-TOF

Additionnal Information

AckA, Acetate kinase A and propionate kinase 2, ECK2290, JW2293, ATGP2844-10 µg, ATGP2844-20 µg, ATGP2844-50 µg, ATGP2844-100 µg, ATGP2844-250 µg, ATGP2844-500 µg, ATGP2844-1 mg, ATGP2844-10, ATGP2844-20, ATGP2844-50, ATGP2844-100, ATGP2844-250, ATGP2844-500, ATGP2844-1

References & Citations

Blattner, F.R., et al. (1997) Science 277 (5331), 1453-1462.; ; Riley, M., et al. (2006) Nucleic Acids Res. 34 (1), 1-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_416799

Uniprot Accession Number

P0A6A3

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA