Recombinant human eEF-2K/EEF2K protein

Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 3 subunit F, EIF3S5, eEF-2 kinase, CaMKIII, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

84.6 kDa (748aa)

Additionnal Information

EEF2K, Eukaryotic elongation factor-2 kinase, eEF-2K, HSu93850, eEF2K, ATGP2837-10 µg, ATGP2837-20 µg, ATGP2837-50 µg, ATGP2837-100 µg, ATGP2837-250 µg, ATGP2837-500 µg, ATGP2837-1 mg, ATGP2837-10, ATGP2837-20, ATGP2837-50, ATGP2837-100, ATGP2837-250, ATGP2837-500, ATGP2837-1

References & Citations

Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:4884-4889; ; Knebel A., et al. (2001) EMBO J. 20:4360-4369

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_037434.1

Uniprot Accession Number

O00418

Species

Human

AA Sequence

MADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPS SNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRY NAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLH AQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQV DIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQG VGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVN QNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSD VTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPR EHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKK IGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAI VGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEA LHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAA EAAMEAMKGR LANQYYQKAE EAWAQMEE