Recombinant e.coli glk protein

Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glucokinase, ECK2384, JW2385

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.1 kDa (344aa) confirmed by MALDI-TOF

Additionnal Information

Glk, Glucokinase, ECK2384, JW2385, ATGP2810-10 µg, ATGP2810-20 µg, ATGP2810-50 µg, ATGP2810-100 µg, ATGP2810-250 µg, ATGP2810-500 µg, ATGP2810-1 mg, ATGP2810-10, ATGP2810-20, ATGP2810-50, ATGP2810-100, ATGP2810-250, ATGP2810-500, ATGP2810-1

References & Citations

Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4) : 320-30.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_416889

Uniprot Accession Number

P0A6V8

Species

E.coli

AA Sequence

MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL