Recombinant human PKA C beta/PRKACB protein

PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. Recombinant human PRKACB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein kinase cAMP-activated catalytic subunit beta, Protein kinase, cAMP-dependent, Beta catalytic subunit, PKACb

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

48.6 kDa (421aa)

Additionnal Information

PRKACB, Protein kinase cAMP-dependent catalytic beta, Protein kinase, cAMP-dependent, catalytic, beta, PKACB, ATGP2666-10 µg, ATGP2666-20 µg, ATGP2666-50 µg, ATGP2666-100 µg, ATGP2666-250 µg, ATGP2666-500 µg, ATGP2666-1 mg, ATGP2666-10, ATGP2666-20, ATGP2666-50, ATGP2666-100, ATGP2666-250, ATGP2666-500, ATGP2666-1

References & Citations

Wu K.-J., et al. (2002) Oncogene. 21:7872-7882; ; Lignitto L., et al. (2011) Nat. Cell Biol. 13:412-422;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_891993

Uniprot Accession Number

P22694

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAYREP PCNQYTGTTT ALQKLEGFAS RLFHRHSKGT AHDQKTALEN DSLHFSEHTA LWDRSMKEFL AKAKEDFLKK WENPTQNNAG LEDFERKKTL GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F