Recombinant human Dynactin Subunit 2/DCTN2 protein
DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Dynactin subunit 2 isoform 1, Dynactin subunit 2 isoform 1, Dynactin 2 (p50), DCTN50, DYNAMITIN, RBP50
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.2 kDa (429aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Echeverri C.J., Paschal B.M. et al. (1996), J. Cell Biol. 132:617-633
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006391.1
Uniprot Accession Number
Q13561
Species
Human
AA Sequence
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