Recombinant human Complement Factor B protein

CFB encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. Recombinant human CFB-Bb protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Complement factor B, AHuS4, BF, BFD, CFAB, FB, FBI12, GBG, H2-Bf, PBF2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

59.4 kDa (528aa)

Additionnal Information

Complement Factor B, complement factor B, AHuS4, BF, BFD, CFAB, FB, FBI12, GBG, H2-Bf, PBF2, ATGP2479-10 µg, ATGP2479-20 µg, ATGP2479-50 µg, ATGP2479-100 µg, ATGP2479-250 µg, ATGP2479-500 µg, ATGP2479-1 mg, ATGP2479-10, ATGP2479-20, ATGP2479-50, ATGP2479-100, ATGP2479-250, ATGP2479-500, ATGP2479-1

References & Citations

Wu, L., et al. (2013) Invest. Ophthalmol. Vis. Sci. 54 (1), 170-174; ; Thakkinstian, A., et al. (2012) Am. J. Epidemiol. 176 (5), 361-372;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001701

Uniprot Accession Number

P00751

Species

Human

AA Sequence

KIVLDPS GSMNIYLVLD GSDSIGASNF TGAKKCLVNL IEKVASYGVK PRYGLVTYAT YPKIWVKVSE ADSSNADWVT KQLNEINYED HKLKSGTNTK KALQAVYSMM SWPDDVPPEG WNRTRHVIIL MTDGLHNMGG DPITVIDEIR DLLYIGKDRK NPREDYLDVY VFGVGPLVNQ VNINALASKK DNEQHVFKVK DMENLEDVFY QMIDESQSLS LCGMVWEHRK GTDYHKQPWQ AKISVIRPSK GHESCMGAVV SEYFVLTAAH CFTVDDKEHS IKVSVGGEKR DLEIEVVLFH PNYNINGKKE AGIPEFYDYD VALIKLKNKL KYGQTIRPIC LPCTEGTTRA LRLPPTTTCQ QQKEELLPAQ DIKALFVSEE EKKLTRKEVY IKNGDKKGSC ERDAQYAPGY DKVKDISEVV TPRFLCTGGV SPYADPNTCR GDSGGPLIVH KRSRFIQVGV ISWGVVDVCK NQKRQKQVPA HARDFHINLF QVLPWLKEKL QDEDLGFL