Recombinant human ACOT11 protein
BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Recombinant human BFIT protein, fused to His-tag at N-terminus, was expressed in E. coli
Product Specifications
Product Name Alternative
Thioesterase adipose associated isoform BFIT2, Acyl-CoA thioesterase 11, THEA, STASTARD14, BFIT, KIAA0707, BFIT1, THEM1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.9 kDa (268aa)
Additionnal Information
References & Citations
Thorsell AG, Lee WH., et al. (2011) Plos One 6 p.19521
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_671517
Uniprot Accession Number
Q8WXI4
Species
Human
AA Sequence
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