Recombinant human GAGE2D protein
GAGE2D, as known as G antigen 2D, belongs to a family of proteins organized in clustered repeats. They have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. The first GAGE nomenclature was based on identified mRNA sequences, but the high identity of the GAGE members made impossible to separate products of paralogous genes from polymorph products. Recombinant human GAGE2D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
G antigen 2D, CT4.8, GAGE-2D, GAGE-8, GAGE8
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15.2 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Backer O. et al. (1999) Cancer Res. 59:3157-3165; ; Gjerstorff MF. et al. (2008) Tissue Antigens. 71:187-192.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_001091877
Uniprot Accession Number
Q9UEU5
Species
Human
AA Sequence
MSWRGRS TYRPRPRRYV EPPEMIGPMR PEQFSDEVEP ATPEEGEPAT QRQDPAAAQE GEDEGASAGQ GPKPEADSQE QGHPQTGCEC EDGPDGQEMD PPNPEEVKTP EEGEKQSQC
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