Recombinant human SPINK7 protein
Serine peptidase inhibitor Kazal type 7, also known as SPINK7, contains 1 Kazal-like domain and is probably serine protease inhibitor. Recombinant human SPINK7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Serine peptidase inhibitor Kazal type 7, ECG2, ECRG2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
9.6 kDa (89aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Clark H.F., et al. (2003) Genome Res. 13:2265-2270; ; Feng Y., et al. (2012) J. Biomol. NMR. 53:65-70
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF, 50 mM imidazole
Scientific Category
Signal Transduction
NCBI Accession Number
NP_115955.1
Uniprot Accession Number
P58062
Species
Human
AA Sequence
SEAASLS PKKVDCSIYK KYPVVAIPCP ITYLPVCGSD YITYGNECHL CTESLKSNGR VQFLHDGSC
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