Recombinant human DNA polymerase epsilon 3/POLE3 protein

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. POLE3 has been shown to interact with SMARCA5. Recombinant human POLE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA polymerase epsilon 3 accessory subunit (approved) /CHRAC2, Histone fold protein CHRAC17, DNA polymerase epsilon p17 subunit, Chromatin accessibility complex 17, Arsenic transactivated protein, Chromatin accessibility complex subunit 2, AsTP, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.2 kDa (170aa) confirmed by MALDI-TOF

Additionnal Information

YBL1, POLE3, p17, DNA polymerase epsilon subunit 3, CHRAC17, CHARAC17, DNA polymerase epsilon 3 accessory subunit (approved) /CHRAC2, Histone fold protein CHRAC17, DNA polymerase epsilon p17 subunit, Chromatin accessibility complex 17, Arsenic transactivated protein, Chromatin accessibility complex subunit 2, AsTP, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, ATGP2141-10 µg, ATGP2141-20 µg, ATGP2141-50 µg, ATGP2141-100 µg, ATGP2141-250 µg, ATGP2141-500 µg, ATGP2141-1 mg, ATGP2141-010, ATGP2141-020, ATGP2141-050, ATGP2141-100, ATGP2141-250, ATGP2141-500, ATGP2141-01M

References & Citations

Bolognese F, Imbriano C, et al. (2000) . Nucleic Acids Res. 28 (19) :3830-8.; ; Li Y, Pursell ZF, et al. (2000) . J Biol Chem. 275 (30) :23247-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_059139

Uniprot Accession Number

Q9NRF9

Species

Human

AA Sequence

MAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN