Recombinant human CD74 protein

HLA class II histocompatibility antigen gamma chain isoform b, also known as CD74, is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. It is upregulated in several cancers and is also expressed by nonimmune cells during inflammation. Recombinant human CD74 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA, II

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.6 kDa (183aa) confirmed by MALDI-TOF

Additionnal Information

CD74, ATGP2104-10 µg, ATGP2104-20 µg, ATGP2104-50 µg, ATGP2104-100 µg, ATGP2104-250 µg, ATGP2104-500 µg, ATGP2104-1 mg, ATGP2104-10, ATGP2104-20, ATGP2104-50, ATGP2104-100, ATGP2104-250, ATGP2104-500, ATGP2104-1

References & Citations

Wilson K M., et al. (1993) Immunology. 79:331-335; ; Henne C., et al. (1995) Immunology. 84:177-182.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_004346.1

Uniprot Accession Number

P04233

Species

Human

AA Sequence

QQQGRLD KLTVTSQNLQ LENLRMKLPK PPKPVSKMRM ATPLLMQALP MGALPQGPMQ NATKYGNMTE DHVMHLLQNA DPLKVYPPLK GSFPENLRHL KNTMETIDWK VFESWMHHWL LFEMSRHSLE QKPTDAPPKE SLELEDPSSG LGVTKQDLGP VPM