Recombinant human CD1b protein
CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This protein contains 1 Ig-like (immunoglobulin-like) domain. CD1B is antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. Recombinant human CD1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
T-cell surface glycoprotein CD1b, CD1, CD1A, R1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.2 kDa (310aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Shamshiev A., et al. (2000) Immunity 13:255-264 ; ; Winau F., et al. (2004) Nat. Immunol. 5:169-174;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_001755
Uniprot Accession Number
P29016
Species
Human
AA Sequence
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