Recombinant human TFB2M protein
TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Recombinant human TFB2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Dimethyladenosine transferase 2 mitochondrial, Dimethyladenosine transferase 2, mitochondrial, Hkp1, mtTFB2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.8 kDa (401aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Litonin, D., et al. (2010) J. Biol. Chem. 285 (24), 18129-18133; ; Norrbom, J., et al. (2010) Acta Physiol (Oxf) 198 (1), 71-79.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_071761.1
Uniprot Accession Number
Q9H5Q4
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Mouse Monoclonal CD8 beta Antibody (V45P5C6/G8) [Alexa Fluor 405]
