Recombinant human THG1L protein
Probable tRNA (His) guanylyltransferase, also known as THG1L, belongs to the tRNA (His) gyanylytransferase family. Localized to the cytoplasm, THG1L is also found near the nuclear membrane and is expressed in many tissues, including liver and lung. upon DNA damage, THG1L is phosphorylated, most likely by ATM or ATR. It is expressed in a cell cycle-dependent manner and may be involved in cell cycle progression and cell proliferation. Recombinant human THG1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Probable tRNA (His) guanylyltransferase, ICF45
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34 kDa (292aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Guo D., et al. (2004) J Biol Chem. 279 (51) : 53498-505.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_060342
Uniprot Accession Number
Q9NWX6
Species
Human
AA Sequence
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