Recombinant human eIF-4H/EIF4H protein
Eukaryotic translation initiation factor 4H, also known as EIF4H, is a 248 amino acid protein that localizes to the perinuclear region of the cytoplasm and is expressed as two isoforms, designated short and long. EIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A and EIF4B. EIF4H was further shown to stimulate the initial rate and extent of EIF4A-mediated mRNA secondary structure unwinding. Defects in the gene encoding EIF4H are associated with Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities. Recombinant human EIF4H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 4H, eIF-4H, WBSCR1, WSCR1, Williams-Beuren syndrome chromosome region 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.9 kDa (272aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Richter Cook N J., et al. (1998) J Biol Chem. 273:7579-7587.; ; Doepker R C., et al. (2004) J Virol. 78: 4684-4699.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_071496
Uniprot Accession Number
Q15056
Species
Human
AA Sequence
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